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Image Search Results
Journal: Scientific reports
Article Title: Novel anticancer agent, SQAP, binds to focal adhesion kinase and modulates its activity.
doi: 10.1038/srep15136
Figure Lengend Snippet: Figure 3. SPR analysis of the binding between SQAP and target proteins. Sensorgrams obtained by SPR analysis of SQAP (left) and SQP (right) with the candidate proteins: (A) SCP-2 KD 15.6 ± 2.2 μ M, (B) MFE-2 KD 14.2 ± 1.79 μ M, (C) ADRM1 KD 9.65 ± 2.91 μ M, (D) HR23B, (E) FAK KD 3.24 ± 0.21 μ M. The candidate proteins were immobilized onto the surface of a CM5 sensor chip. A solution of SQAP or SQP at variable concentrations was injected to generate result binding responses (RU) recorded as a function of time (sec). The results were analyzed using BIA evaluation 4.1.
Article Snippet: The proteins were first immobilized on a CM5 sensor chip of the
Techniques: Binding Assay, Injection
Journal: Molecular Therapy. Methods & Clinical Development
Article Title: Efficient inhibition of tumor angiogenesis and growth by a synthetic peptide blocking S100A4-methionine aminopeptidase 2 interaction
doi: 10.1038/mtm.2015.8
Figure Lengend Snippet: Interaction of S100A4 with the NBD peptide. ( a ) SPR analyses. The SBD peptide was injected over the captured GST-S100A4 and GST in the presence or absence of 1 mmol/l CaCl 2 . The data shown are from a duplicated experiment. ( b ) In vitro binding assay. MetAP2 was incubated with either GST or the GST/S100A4 fusion protein in the presence of 1 mmol/l EDTA or 1 mmol/l CaCl 2 . Peptide was added at a 25-fold molar excess of the amount of MetAP2. ( c ) FRET analysis. The graph shows FRET efficiencies (%) calculated from the acceptor-photobreaching analyses in HeLa cells expressing indicated protein pairs. Green bars are negative controls, while orange bars are the positive control (tandem-linked ECFP+EYFP) and experimental groups. n = 8–11. ** P < 0.01; *** P < 0.001.
Article Snippet: The binding of the SBD peptide to S100A4 was examined by Surface plasmon resonance (SPR) with a Biacore 3000 biosensor and
Techniques: Injection, In Vitro, Binding Assay, Incubation, Expressing, Positive Control